Objective: To study the value of myocardial contrast echocardiography (MCE) and color Doppler coronary flow imaging (CDCFI) in transcoronary ablation of septal hypertrophy (TASH) for hypertrophic obstructive cardiomyopathy (HOCM) patients. Methods: A total of 94 HOCM patients who underwent evaluation for TASH were divided into three groups: Group 1 (n=32): myocardial contrast echocardiography (MCE) alone; Group 2 (n=37): MCE during procedure combined with color Doppler coronary flow imaging (CDCFI); Group 3 (n=25): MCE during and post procedure combined with CDCFI. Results: In group 1, 1 patient had a 3rd degree AV block, 7 patients were found no appropriate vessels for ablation during procedure, 2 patients were found to have suboptimal ablation during post-op follow up. In Group 2, no serious complications occurred, 4 patients had no suitable vessel for ablation founded by CDCFI before procedure. 3 patients were found to have suboptimal ablation during follow up. In Group 3, no serious complications occurred, 2 patients had no suitable vessel for ablation founded by CDCFI before procedure, and 2 patients were found to have suboptimal ablation immediately after the procedure. No patients were found to have suboptimal ablation during follow up. In follow up, 5 of 94 patients developed post procedural LVOT gradients detected by MCE. Conclusions: The application of CDCFI and MCE assists in the selection of suitable patients and correct vessels for ablation, in the identification of post procedural LVOT gradients, in a reduction in the incidence of complications in patients undergoing TASH.