Effect of Hematoporphyrin Activated by Ultrasound on Anoikis Resistancein HepG2 Hepatocarcinoma Cell

  • Prof Haitao Ran, Institute of Ultrasound Imaging, Second Affiliated Hospital of Chongqing Medical University, China
  • Prof Zhigang Wang, Institute of Ultrasound Imaging, Second Affiliated Hospital of Chongqing Medical University, China
  • Dr Juan Cheng, Institute of Ultrasound Imaging, Second Affiliated Hospital of Chongqing Medical University, China
  • Dr Pan Li, Institute of Ultrasound Imaging, Second Affiliated Hospital of Chongqing Medical University, China

    • Objective: To investigate the effect of hematoporphyrin activated by ultrasound on anoikis resistance in HepG2 hepatocacinoma cells.
    Methods: The HepG2 cell suspension was randomly divided into four groups as follows: the cells in hematoporphyrin group incubated in culture solution containing 40 microliters (µl) of
    hematoporphyrin for 30s;those in ultrasound group irradiated by 1.0 MHz, 1.5 Wcm2 ultrasound for 30s; those in hematoporphyrin and ultrasound group treated by both, and others without treatment as the control group. After treatment, a number of cells were cultured in soft agar for two weeks to observe their growth ability during loss of cell adhesion. The other cells were cultured in plates covered with poly-hydroxy ethylmethacrylate (poly-HEMA) for 15 hours and the anoikis was detected by DNA laddering assay and Annexin V-FITCPI double staining.
    Results: In hematoporphyrin and ultrasound group no cell colony was found in soft agar, the DNA ladder was observed and the apoptosis rate was 14.89±2.82%. Differences with other three groups were significant.
    Conclusions: Hematoporphyrin activated by ultrasound can inhibit the anoikis resistance in HepG2 hepatocacinoma cells.
    Keywords: Hepatocarcinoma,Ultrasound,Hematoporphyrin,Anoikis.