Cholangiolocellular adenocacinoma (CLC), a subtype of cholangiocellular carcinoma (CCC), is extremely rare, which is considered to originate from canals of Hering ductules potentially to differentiate in either direction: hepatocyte or bile duct cells. We report a 64-year old man who has been suffering from Diabetes Mellitus, liver dysfunction possibly due to alcoholic steatohepatitis, brain infarction and spinal cord stenosis. The liver mass was incidentally found by the abdominal ultrasonography (US) at the screening test preparing for operation of spinal cord: a 2cm irregular round mass with well-defined border and peripheral hypoechoic rim, in the bright liver with dull edge. On the contrast enhanced harmonic US (CE-US) with Sonazoid, the mass showed rim-like enhancement from the peripheral to the central portion with partial central sparing, in the early vascular phase, and washout in the late vascular phase (1min later), which hemodynamic imaging pattern was compatible with CCC, most probably. Tumor markers of CEA and CA19-9 were slightly elevated: 7 ng/ml and 42.2 U/ml, respectively. The abdominal CT demonstrated peripheral rim enhancement in the early vascular phase, suggesting metastatic tumor, CCC or inflamatory pseudotumor. The 18-FDG-PET revealed hot spot at the mass, but not confirmed diagnosis. Upper and lower gastrointestinal tract endoscopic examinations for metastatic survey were normal. US-guided fine needle biopsy was performed for final diagnosis, and the histopathological findings confirmed CLOC with central fibrosis, peripheral replacing growth, and not involving portal branches at periphery. We thought that CE-US could be the most useful modality for CCC and CLOC.